Bioremediation of hydrocarbons in wastewater with mixed culture of microorganisms: case car washers Puyango

Authors

  • Judit García Universidad UTE
  • Daniel Peñafiel Heredia Universidad UTE
  • Remberto Rodríguez Universidad UTE

DOI:

https://doi.org/10.29019/enfoqueute.v10n1.312

Keywords:

bioremediation, hydrocarbons, wastewater, microorganisms, removal.

Abstract

Wastewater from car washers and lubricators contain high levels of hydrocarbons. These are discharged into the public sewer system without proper treatment and not complying with the regulations. In this study was evaluated the process of bioremediation of total hydrocarbons in Puyango’s wastewater, using bioaugmentation treatment, which means, applying hydrocarbons-degrading microorganisms. First, a characterization of the wastewater was carried out, and the content of total petroleum hydrocarbons (TPH) and the flow rate was determined for seven days. A mixture of bacteria: Acinetobacter sp., Pseudomonas sp. y Mycobacterium sp was applied in solid form (80 g tablet) with a minimum concentration of 4 x 108 CFU / ml soluble in water, using 2 tablets during 30 days of treatment. The concentration of the TPH was determined weekly, plotting the temporal variation and quantifying the % of removal. The results indicate that the technique of bioaugmentation was effective (p = 0.003) in the removal of the TPH, obtaining percentages above 86 %. The time of greatest removal of TPH was obtained in the third week of treatment with 92 %. In addition, COD removals were obtained –40 %, Oils and fats - 50%, Surfactants –43 % thus fulfilling the current regulations for discharge to the public sewer system for the TPH.

Downloads

Download data is not yet available.

References

Acuerdo Ministerial 097 de la Reforma al Texto Unificado de Legislación Segundaria (2015).
Acuña, A., Tonín N., Díaz V., Pucci G.N., Pucci O.H. (2011). Optimización de un sistema de biorremediación de hidrocarburos a escala de laboratorio. Ingeniería Investigación y Tecnología. Vol. XIII, Núm. 1, 2012, 105-112. ISSN 1405-7743 FI-UNAM.
Adams Schroeder, R., Domínguez Rodríguez, V., & García Hernández, L. (1999). Bioremediation Potential of Oil Impacted Soil and Water in the Mexican Tropics. Tabasco: Universidad Juárez Autónoma de Tabasco.
Albarracín, P. (2006). Tribología y Lubricación Industrial y Automotriz. Tomo I Cuarta Edición. Colombia.
APHA. (1999). Standard Methods for the Examination of Water and Wastewater. EEUU: American Public Health Association, American Water Works Association, Water Environment Federation.
Araujo, I., Gómez, A., Barrera , M., Angulo, N., Morillo, G., Cardenas, C., & Herrera, L. (2008). Surfactantes biológicos en la biorremediación de aguas contaminadas con crudo liviano. INCI vol 33 No4. Obtenido de Scielo: http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0378-18442008000400004#fig3
Ferrera, R et al. (2006). Procesos de biorremediación de suelo y agua. Revista Latinoamericana de Microbiologia, Vol 48 No 2. 179-187.
Fortunato, M., Rossi, S., Korol, S., & D'aquino, M. (1997). Eficiencia de la Degradación Microbiana de Tensioactivos:. Buenos Aires: Cátedra de Higiene y Sanidad. Facultad de Farmacia y Bioquínzica.
GAD Provincial Santo Domingo de los Tsachilas, (2015). Lista Taxativa de las Actividades Económicas de la Provincia. Santo Domingo de los Tsachilas.
García Cruz, U., & Aguirre Macedo, M. (2014). Biodegradación de petróleo por bacterias: algunos casos de estudio en el Golfo de México. Mérida. Microbiología 641-652
González, A. (2014). Manual Piragüero-Medición del Caudal. Medellin: LibroArte.
Martínez, A et al. (2011) Biorremediación de suelo contaminado con hidrocarburos empleando lodos residuales como fuente alterna de nutrientes. Rev. Int. Contam. Ambie 27(3) 241-252.
Rivera, P et al. (2018) Bioestimulación y biorremediación de recortes de perforación contaminados con hidrocarburos. Rev. Int. Contam. Ambie. 34 (2) 249-262. DOI: 10.20937/RICA.2018.34.02.06
Romaniuk, R.; Brandt, J; Rios, P & Giuffré, L. (2007) Atenuación natural y remediación inducida en suelos contaminados con hidrocarburos. CI Suelo (Argentina) 25(2): 139-149.
Ron, E., & Rosenberg, E. (2002). Biosurfactants and oil bioremediation. Israel: Elsevier Science Ltd.
Salleh, A.B., F.M. Ghazali, R.N.A. Rahman, & M. Basri., (2003). Bioremediation of petroleum hydrocarbon pollution. Indian Journal of Biotechnology, 2: 411-425.
Solanas, A. (2009). La biodegradación de hidrocarburos y su aplicación en la biorremediacion de suelos. Estudios en la Zona no Saturada del Suelo. Vol IX, O. Barcelona.

Downloads

Published

2019-03-29

How to Cite

García, J., Peñafiel Heredia, D., & Rodríguez, R. (2019). Bioremediation of hydrocarbons in wastewater with mixed culture of microorganisms: case car washers Puyango. Enfoque UTE, 10(1), pp. 185 – 196. https://doi.org/10.29019/enfoqueute.v10n1.312

Issue

Vol. 10 No. 1 (2019)

Section

Environment

License

The articles and research published by the UTE University are carried out under the Open Access regime in electronic format.  This means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access. By submitting an article to any of the scientific journals of the UTE University, the author or authors accept these conditions.

The UTE applies the Creative Commons Attribution (CC-BY) license to articles in its scientific journals. Under this open access license, as an author you agree that anyone may reuse your article in whole or in part for any purpose, free of charge, including commercial purposes. Anyone can copy, distribute or reuse the content as long as the author and original source are correctly cited. This facilitates freedom of reuse and also ensures that content can be extracted without barriers for research needs.

 

This work is licensed under a Creative Commons Attribution 3.0 International (CC BY 3.0).

 

The Enfoque UTE journal guarantees and declares that authors always retain all copyrights and full publishing rights without restrictions [© The Author(s)]. Acknowledgment (BY): Any exploitation of the work is allowed, including a commercial purpose, as well as the creation of derivative works, the distribution of which is also allowed without any restriction.