Bromatological characterization and evaluation of the antimi- crobial activity of Ecuadorian banana peel (Musa paradisiaca)
The bromatological composition of banana peel (Musa paradisiaca) produced in Ecuador was determined and the fractions and phytochemical groups responsible for its antimicrobial activity were identified. The bromatological analysis determined a humidity of 88.94% and 11.06% of total solids (1.55% of ash, 0.47% of fat, 0.74% of protein, 0.87% of fiber and 8.3% total carbohydrates). To obtain the fractions, two methods of maceration were applied. The first used four solvents of different polarities, such as petroleum ether, chloroform, ethyl acetate and water. The extracts were subjected to phytochemical screening tests that identify the presence of fatty compounds, sterols, triterpenes, saponins and tannins. The second method used 70% ethanol as solvent and five fractions were generated. Tests of antimicrobial activity by the agar diffusion method were performed in Staphylococcus aureus and Escherichia coli using the crude ethanolic extract and its fractions, microbiological activity was evidenced. Phytochemical screening assays and chromatography were performed on each fraction and extract identifying the main groups of compounds responsible for the antimicrobial activity.
A.O.A.C. Association of Analytical Chemist. (2000). “Oficial Methods of Analysis”. Inc. Washington, D.C. E.U.A.
Chinnappan, S. et al. (2013). “Evaluation of microbial potential of different colored banana peels”. International Journal of Preclinical & Pharmaceutical Research. 4(2), 62-64.
Clinical and Laboratory Standards Institute. (2012, 01). MIC testing Volume 32. Number 2. Recuperado de http://antimicrobianos.com.ar/ATB/wp-content/uploads/2012/11/04-DETERMINACION-DE-LA-SENSIBILIDAD-METODO-DE-DILUCION-2012.pdf (accedido el 07/02/2018).
Guil-Guerrero, J. et al. (2016)a. “Antimicrobial activity of plant-food by-products: A review focusing on the tropics”. Livestock Science, 189, 32-39.
Guil-Guerrero, J. et al. (2016)b. “Plant Foods By-Products as Sources of Health-Promoting Agents”. Agronomy Journal, 108, 1-16.
Guil-Guerrero, J. et al. (2016)c. “Plant-food by-products to improve farm-animal health”. Animal Feed Science and Technology, 1-15.
MAGAP. (2014). Boletin Situacional Banano. Recuperado de http://sinagap.agricultura.gob.ec/phocadownloadpap/cultivo/2014/aboletin-situacional-banano-2014-actualizado.pdf (accedido el 07/02/2018)
Matos, F. (2009). Introdução à Fitoquímica Experimental (3 ed.). Fortaleza_Brasil. Edicóes UFC.
Mordi, R. et al. (2016). “Identification by GC-MS of the Components of oils of Banana Peels Extract, Phytochemical an Antimicrobial Analyses”. Research Journal of Phytochemistry, 10, 39-44.
Niamah, A. (2014). “Determination, identification of bioactive compounds extracts from yellow banana peels and used in vitro as antimicrobial”. International Journal of Phytomedicine, 6, 625-632.
Pavarini , D., Pavarini, S., Niehues, M., & Lopes, N. (2012 ). “Exogenous influences on plant secondary metabolite levels”. Animal Feed Science and Technology, 176 (1-4) pp: 5-16.
PROECUADOR. (2016). Sector Analysis Banano 2016. Recuperado de http://www.proecuador.gob.ec/pubs/banano-sector-analysis/ (accedido el 07/02/2018)