Physicochemical properties and microbial group behavior of postharvest peruvian cocoa bean (Theobroma cacao L.)
DOI:
https://doi.org/10.29019/enfoqueute.v11n4.602Keywords:
Food safety, fungal contamination, microorganisms, Peru, sun-dryingAbstract
Cocoa is used in the manufacturing of different products. Fermenting microorganisms such as yeast and bacteria use the high nutrient content of cocoa. This research evaluated physicochemical properties and microbial group behavior of fresh cocoa bean and four treatments to compare among them (A, A-farmer, B, and B-farmer). Moisture content, mold and yeast count, and Salmonella were determined in fresh cocoa beans and treatments. The results of fresh cocoa beans were moisture content of 56.30 ± 0.02 % and microbiological count below control. Treatments results were moisture content of 3.10 ± 0.02 %, 10.30 ± 0.20 %, 4.80 ± 0.00 %, and 11.40 ± 0.20 % (p ≤ 0.05), mold and yeast count were statistically similar (p > 0.05). Salmonella was not detected in any of the treatments. These findings indicate that regardless of post-harvest process, there is no fungal contamination in cocoa when carrying out good manufacturing practices.
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Afoakwa, E.O.; Budu, A.S.; Mensah-Brown, H. et al. (2014). Changes in Biochemical and Physico-chemical Qualities During Drying of Pulp Preconditioned and Fermented Cocoa (Theobroma cacao) Beans. Journal of Nutrional Health & Food Science 2(3), 1–8. doi: 10.15226/jnhfs.2014.00121
Afoakwa, E.O.; Paterson, A.; Fowler, M. et al. (2008). Flavor Formation and Character in Cocoa and Chocolate: A Critical Review. Critical Reviews in Food Science and Nutrition 48(9), 840–857. doi: 10.1080/10408390701719272.
Aprotosoaie, A. C.; Luca, S. V.; Miron, A. (2016). Flavor Chemistry of Cocoa and Cocoa Products – An Overview. Comprehensive Reviews in Food Science and Food Safety 15, 73-91. doi: 10.1111/1541-4337.12180
Ardhana, M. M., Fleet, G.H. (2003). The Microbial Ecology of Cocoa Bean Fermentations in Indonesia. International Journal of Food Microbiology 86, 87-99. doi: 10.1016/S0168-1605(03)00081-3
Asep, E. K.; Jinap, S.; Tan, T. J. et al. (2008). The Effect of Particle Size, Fermentation and Roasting of Cacao Nibs on Supercritical Fluid Extraction of Cacao Butter. Journal Food Engineering 85, 450-458. doi: 10.1016/j.jfoodeng.2007.08.008
AOAC. (2016). Official Methods of Analysis of the AOAC International. In William Horwitz and George W. Latimer, Jr (eds.). Method 931.04, 972.15, 970.22, 920.75a, 970.21. (20o. ed.). Vol. II. . Maryland, USA.
Belitz, H .D.; Grosch, W.; Schieberle, P. (2009). Food Chemistry. (4o. revised and extended edition). Springer.
Burndred, F. (2009). Food Safety in Chocolate Manufacture and Processing. In: Beckett, S. (eds). Industrial Chocolate Manufacture and Use. Wiley-Blackwell. York, United Kingdom, pp. 530-550. doi: 10.1002/9781444301588
Castro, M. D. R.; Hernández, J. A.; Marcilla, S. et al. (2016). Efecto del contenido de grasa en la concentración de polifenoles y capacidad antioxidante de Theobroma cacao L. “CACAO”. Ciencia e Investigación 19(1), 19-23.
Contreras, C. L.; Ortiz de Bertorelli, L.; Graziani de Fariñas, L. et al. (2004). Cocoa Fermenters Used by Farmers of Cumboto,Venezuela. Agronomía tropical 54(2), 219-232.
Copetti, M. V.; Iamanaka, B.T.; Frisvad, J.C. et al. (2011a). Aflatoxigenic Fungi and Aflatoxin in Cacao. International Journal of Food Microbiololy 148,141-144. doi: 10.1016/j.ijfoodmicro.2011.05.020
Copetti, M. V.; Iamanaka, B. T.; Frisvad, J.C. et al. (2011b). Mycobiota of Cacao: From farm to Chocolate. Food Microbiololy 28, 1499-1504. doi: 10.1016/j.fm.2011.08.005
Copetti, M. V.; Iamanaka, B.T.; Pitt, J. I. et al. (2014). Fungi and Mycotoxins in cacao: From farm to chocolate. International Journal of Food Microbiology 178,13-20. doi: 10.1016/j.ijfoodmicro.2014.02.023
Cordier, J. L. (2000). Sugars, Honey, Cacao, Chocolate and Confectionery Products. In: Lund BM, Baird-Parker Tc, Gould GW (eds.). The Microbiological Safety and Quality of Food 1, 941-959.
Chaves-López, C.; Serio, A.; Grande-Tovar, C. D. et al. (2014). Traditional Fermented Foods and Beverages from a Microbiological and Nutritional Perspective: The Colombian Heritage. Comprehensive review in food science and food safety 13, 1031-1048. doi: 10.1111/1541-4337.12098
Chávez-Rivera, R. E.; Ordoñez-Gómez, E.S. (2013). Polifenoles totales, antocianinas y capacidad antioxidante (DPPH y ABTS) durante el procesamiento de licor y polvo de cacao. ECI-Perú, 10(1), 42-50.
Chire, G. C.; Valdivia, R. A.; Ureña, M. O. (2014). Ocratoxin A in Cocoa and Derivatives. Preventive Measures. Ciencia e Investigación 17(1), 9-15.
Fonseca, M. J.; Tavares, F. (2011). The Bactericidal Effect of Sunlight. The American Biology Teacher 73(9), 548-552. doi: 10.1525/abt.2011.73.9.8
Fowler, M.S.; Coutel, F. (2017). Cocoa Beans: From Tree to Factory. In: Beckett, S., Fowler, MS., Ziegler, GR. (eds). Beckett´s Industrial Chocolate Manufacture and Use. (5º. ed.), p.18. Wiley-Blackwell. York, United Kingdom.
Icmsf (2000). Microbiología de alimentos 1: Significado y método de elaboración. (2º. ed.), p. 454. Editorial Acribia S. A. Zaragoza, España.
Inacal (2016). NTP ISO 2451. Granos de cacao. Especificaciones. (4º. ed.). Lima.
Inen (2006). NTE 176:2006. Cacao en grano. Requisitos. Lima.
Jay, J. M. (2000). Modern Food Microbiology. (6o. ed.), p. 679. Gaithersburg (MD): Aspen,.
Komitopoulou, E.; Peñaloza, W. (2009). Fate of Salmonella in Dry Confectionery Raw Materials. Journal of Applied Microbiology 106(6),1892-1900. doi: 10.1111/j.1365-2672.2009.04144.x
Lippi, D. (2013). History of the Medical Use of Chocolate. In Ross Watson, R., Preedy, RB., Zibai, S. (eds.). Chocolate in health and nutrition. Humana Press. Series Editor: Adrianne Bendich.
Loo-Miranda, J. L. M.; Chire-Fajardo, G. C.; Ureña-Peralta, M. O. (2020). Modeling of the Variation of the Electrical Conductivity of the Leachate of Cocoa with Different Fermentation Index. Enfoque UTE 11(3), 46-54.
Ministerio de Salud (Minsa). (2008). Norma sanitaria que establecen los criterios microbiológicos de calidad sanitaria e inocuidad para los alimentos y bebidas de consumo humano. N591, Lima, pp. 19-20.
Mozzi, F.; Ortiz, M. E.; Bleckwedel, J. et al. (2013). Metabolomics as a Tool for the Comprehensive Understanding of Fermented and Functional Foods with Lactic Acid Bacteria. Food Research International 54(1), 1152–1161. doi:10.1016/j.fo odres.2012.11.010
Nascimento, M. D. S.; Pena, P. O.; Brum, D. M. et al. (2013). Behavior of Salmonella during Fermentation, Drying and Storage of Cocoa Beans. International Journal of Food Microbiology 167(3), 363-368. doi: 10.1016/j.ijfoodmicro.2013.10.003
Papalexandratou, Z.; Lefeber, T.; Bahrim, B. et al. (2013). Hanseniaspora opuntiae, Saccharomyces cerevisiae, Lactobacillus fermentum, and Acetobacter pasteurianus Predominate during Well-Performed Malaysian Cacao Bean Box Fermentations, Underlining the Importance of these Microbial Species for a Successful Cacao Bean Fermentation Process. Food Microbiology 35(2), 73-85. doi: 10.1016/j.fm.2013.02.015
Portillo, E.; Graziani De Fariñas, L.; Betancourt, E. (2005). Effect of Postharvest Treatments on Temperature and Fermentation Index in Quality of Porcelana (Theobroma Cacao L.) Criollo Cocoa Bean in the South of Maracaibo Lake. Revista Facultad Agronomia (LUZ) 22(4), 388-399.
Sangronis, E.; Soto, M. J.; Valero, Y. et al. (2014). Cascarilla de cacao venezolano como materia prima para infusiones. Archivos Latinoamericanos de Nutrición 64(2), 123-130.
Sarbu, I.; Csutak, O. (2019). The Microbiology of Cocoa Fermentation. In: Grumezescu, AM., Holban, AM. (eds). Vol. 8. Cafeinatted and Cocoa Based Beverages. The science of beverages. Woodhead publishing, pp. 423-446.
Scott, V. N.; Chen, Y.; Freier, T. A. et al. (2009). Control of Salmonella in Low-moisture Foods. I: Minimizing Entry of Salmonella into a Processing Facility. Food Protection Trends 29(6), 342-353.
Zahouli, GIB.; Tagro-Guehi, S.; Monké-Fac A. Et Al. (2010). Effect of Drying Methods on the Chemical Quality Traits of Cocoa Raw Material. Advance journal of Food Science and Technology 2(4), 184-190.
Zambrano, A.; Gomez, A.; Ramos, G. et al. (2010). Caracterización de parámetros fisicoquímicos de calidad en almendra de cacao criollo, trinitario y forastero durante el proceso de secado. Agronomia Tropical 60(4), 389-396.
Werber, D.; Dreesman, J.; Feil, F. et al. (2005). International outbreak of Salmonella Orannienburg due to German chocolate. BMC Infectious Diseases 5(1), 5-7. doi: 10.1186/1471-2334-5-7
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