Anaerobic horizontal flow reactor with polyethylene terephthalate as support material


  • Marcelo Muñoz Escuela Politécnica Nacional
  • Valeria Fuentes Escuela Politécnica Nacional
  • María Belén Aldás Escuela Politécnica Nacional



anaerobic digestion, horizontal flow reactor, biofilm, wastewater, dairy industry


A pilot anaerobic reactor was installed to remove the organic load of wastewater from dairy industry. It uses a bacterial inoculum previously acclimated to the substrate. It was disposed horizontally and filled with pieces of polyethylene terephthalate (PET), from plastic bottles. The reactor was operated at room temperature, during 100 days, in three phases: 1) the reactor was stabilized with volumetric organic load from 0.013 to 0.500 kg/day.m³; 2) the hydraulic retention time was of 1 day and the volumetric organic load of 3 kg/day.m³; 3) the volumetric organic load was incremented from 4 to 6.6 kg/day.m³ and the hydraulic retention time was 1 day. Organic material removal efficiencies was of 85%, and approximately 75% were obtained in the second and third phase, respectively. The Y value was 0.15, indicating that 0.15 kg of biomass were generated by kg of QDO supplied to the reactor. Finally, the biomass generated inside the reactor was analyzed, obtaining a value of 18868 mg/L, which is a higher value than those of conventional systems.


Download data is not yet available.


Anderson, G.K., Kasapgil, B., Ince, O. (1994). Comparison of porous and non-porous media in upflow anaerobic filters when treating dairy wastewater. Water Research. (28) 7, 1619-1624. doi:10.1016/0043-1354(94)90229-1

Arango O., Sanches, L. (2009). Tratamiento de aguas residuales de la industria láctea en sistemas anaerobios tipo UASB. Facultad de Ciencias Agropecuarias (9) 2, 24-31.

Arango A., Garcés, F. (2007). Tratamiento de aguas residuales de la industria láctea. Producción más limpia, (2) 2, 23-30.

Campos, R. G., Parra, R. A. (2015). Evaluación del comportamiento de un reactor UASB con diferentes cargas orgánicas provenientes de lactosuero. Producción + Limpia, 9(1), 23-30.

Demirel, B., Yenigun, O., Onay, T. T. (2005). Anaerobic treatment of dairy wastewaters: a review. Process Biochemistry, 40(8), 2583-2595. doi:10.1016/j.procbio.2004.12.015

Ince, O. (1998). Potential energy production from anaerobic digestion of dairy wastewater. Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering. (33) 6, 1219-1228. doi:10.1080/10934529809376784

Karadag, D., Köroğlu, O. E., Ozkaya, B., Cakmakci, M. (2015). A review on anaerobic biofilm reactors for the treatment of dairy industry wastewater. Process Biochemistry, 50(2), 262-271. doi:10.1016/j.procbio.2014.11.005

Kolhe, A. S., Ingale, S. R., Bhole, R. V. (2009). Effluent of dairy technology. Shodh Samiksha aur Mulyankan (International Research Journal), 2, 459-461.

Kushwaha, J. P., Srivastava, V. C., Mall, I. D. (2011). An overview of various technologies for the treatment of dairy wastewaters. Critical reviews in food science and nutrition, 51(5), 442-452. DOI:10.1080/10408391003663879

López, P., Harnisth, A. (2016). Electrocoagulación de aguas residuales de la industria láctea. Enfoque UTE, 7(1), 13-21.

Prócel, D., Posligua, P., Banchón, C. (2016). Biodegradación de contaminantes orgánicos de la industria láctea. Enfoque UTE, 7(1), 22-32.

Rodríguez, J., Pérez, D., de la Garza, J. A. R., Garza, Y. (2005). Evaluación cinética de las aguas residuales de la industria láctea en sistemas batch y un reactor UASB. XI Congreso de Biotecnología y Bioingeniería Sociedad Mexicana de Biotecnología y Bioingeniería, Yucatán, México.

Solano J., Rangel, M. (2006). Evaluación operacional de un sistema a escala laboratorio de biopelícula anaerobia soportada para el tratamiento de aguas residuales domésticas (tesis de pregrado). Universidad Industrial de Santander, Bucaramanga, Colombia.

Tawfik, A., Sobhey, M., Badawy, M. (2008). Treatment of a combined dairy and domestic wastewater in an up-flow anaerobic sludge blanke (UASB) reactor followed by activated sludge (AS system). Desalination, (227) 1, 167-177. doi:10.1016/j.desal.2007.06.023

Tikariha, A., & Sahu, O. (2014). Study of characteristics and treatments of dairy industry waste water. Journal of applied & environmental microbiology,2(1), 16-22. DOI:10.12691/jaem-2-1-4

Torres-Sanchez, A. L., Lopez-Cervera, S. J., de la Rosa, C., Maldonado-Vega, M., Maldonado-Santoyo, M., Peralta-Hernandez, J. M. (2014). Electrocoagulation process coupled with advance oxidation techniques to treatment of dairy industry wastewater. International Journal of Electrochemical Science, 9, 6103-6112.

Wang, S., Chandrasekhara Rao, N., Qiu, R. Moletta, R. (2009). Performance and kinetic evaluation of anaerobic moving bed biofilm reactor for treating milk permeate from dairy industry. Bioresource Technology, (100) 23, 5641-5647. doi:10.1016/j.biortech.2009.06.028



How to Cite

Muñoz, M., Fuentes, V., & Aldás, M. B. (2016). Anaerobic horizontal flow reactor with polyethylene terephthalate as support material. Enfoque UTE, 7(2), pp. 97 - 108.